Prostaglandin F 2α and insulin stimulate phosphate uptake and (Na +, K +)ATPase activity in resting mouse fibroblast cultures

Abstract

The (Na +, K +)ATPase transport system in resting 3T3 Swiss mouse fibroblasts is rapidly activated by prostaglandin F 2α and insulin, which initiate DNA synthesis in these cells. Prostaglandin F 2α, but not insulin, promotes a rapid increase in P i uptake which is partially coupled to the Na + pump. This rapid activation of both transport systems occurs by a mechanism which does not require fluctuation of cyclic AMP levels or new protein synthesis. A subsequent protein synthesis-dependent increase in P i uptake is stimulated by insulin and prostaglandin F 2α. These results suggest that different types of control of membrane transport occur during growth stimulation.