Prostaglandin D 2 and prostaglandin E 2 accelerate the recovery of cutaneous barrier disruption induced by mechanical scratching in mice

Abstract

The role of prostaglandins in mechanical scratching-induced cutaneous barrier disruption in mice was investigated. Skin prostaglandins contents were measured after cutaneous barrier function was disrupted by scratching using a stainless-steal wire brush (mechanical scratching), then effects of prostanoids on recovery of cutaneous barrier functions were examined. This mechanical scratching increased transepidermal water loss and skin prostaglandins (prostaglandin D 2, prostaglandin E 2, 6-keto-prostaglandin F 1α and prostaglandin F 2α) contents, count-dependently. Topical application of indomethacin immediately after cutaneous barrier disruption delayed the recovery period of cutaneous barrier disruption. We examined effects of several prostanoids (prostaglandin D 2, prostaglandin E 2, prostaglandin F 2α, prostaglandin I 2 and U46619) on delay of the recovery process of mechanical scratching-induced cutaneous barrier disruption with treatment of indomethacin. Topically applied prostaglandin D 2 and prostaglandin E 2 accelerated the recovery of cutaneous barrier disruption and topical application of prostaglandin J 2, limaprost, sulprostone and ONO-4819, but not 13,14-dihydro-15-keto-prostaglandin D 2, 15-deoxy-Δ 12,14-prostaglandin J 2, 17-phenyl-trinor-prostaglandin E 2 or butaprost had effects on recovery of the cutaneous barrier. These results suggest that prostaglandin D 2 and prostaglandin E 2 accelerate the recovery process of cutaneous barrier disruption caused by mechanical scratching, via specific prostanoid DP 1, EP 3 and EP 4 receptors.