Abstract
We have previously shown (Erman and Raz, 1981; Erman et al, 1982) that mitochondria from rabbit kidney medulla contains prostaglandin synthase activity. Current studies are aimed at localizing this activity in the mitochondrial membrane subfractions. Medullary mitochondria was subfractionated into outer and inner membrane fractions which were characterized by specific enzyme markers. Both outer and inner membrane fractions catalyzed prostaglandin E2 (PGE2) biosynthesis from arachidonic acid. Significantly, the specific activity of prostaglandin synthase in the outer membrane was 9-10 fold higher than in the inner membrane.
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