Prostacyclin protects ischemic reperfused myocardium in the dog by inhibition of neutrophil activation

Abstract

Prostacyclin (PGI 2) and the stable PGI 2 analogue SC39902 (6,9α-epoxy, 5S-fluoro-11α, 15S-dehydroxyprosta-6, 13E-dien-1-oic acid, sodium salt) were studied in anesthetized open-chest dogs subjected to 90 minutes of left circumflex coronary artery (LCCA) occlusion and 6 hours of reperfusion. PGI 2 (50 ng/kg/min, infused into the left atrium) reduced infarct mass by 59% compared to control, but SC39902 (1.5 μg/kg/min) failed to produce a significant reduction in infarct size. Both PGI 2 and SC39902 reduced mean arterial blood pressure, heart rate, and rate-pressure product to the same extent. Regional myocardial blood flow measured with radiolabelled tracer microspheres did not demonstrate an increase in regional blood flow to the ischemic myocardium during the 90 minutes of LCCA occlusion in the PGI 2 and control treatment groups. Canine neutrophils were isolated from whole blood and activated with opsonized zymosan. PGI 2 produced a concentration-dependent inhibition of neutrophil activation as measured by superoxide production in vitro, whereas SC39902 failed to effectively inhibit neutrophil activation. Neutrophil migration into inflammatory skin lesions was effectively attenuated when dogs were pretreated with PGI 2 (50 ng/kg/min, intravenously). Therefore, it is suggested that the cytoprotective effect of PGI 2 during myocardial ischemia and reperfusion is related to an inhibition of neutrophil migration and the production of cytotoxic activated oxygen species.