Abstract
1. 1. Left ventricular slices of male Sprague-Dawley rats were incubated with a fixed concentration of 0.5 μCi/ml 3H-lysine and several concentrations of unlabelled lysine ranging from 0.2 to 5.0 mM in control and prostacylin-treated experiments. The time of incubation ranged from 0.5 to 90 min. 2. 2. Left ventricular slices were cut to have an optimal thickness of 0.47 ± 0.09 mm. 3. 3. Lysine was taken up against a concentration gradient. Saturation was reached at 0.5 mM and steady state accumulation of lysine was attained within 60 min. 4. 4. Prostacyclin in concentrations ranging from 1.2 × 10 −8 to 4.8 × 10 −8 M inhibited lysine transport in left ventricular slices significantly ( P < 0.01).
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