Prostacyclin-independence in beta-adrenoceptor mediated renin release from dog renal cortical slices

Abstract

There is some controversy regarding whether stimulation of renin release by the β-adrenergic system is dependent on prostaglandin (PG) production. We have examined this problem in renal cortical slices of the dog and have obtained the following results: (1) Isoproterenol (4×10 −6 M) stimulated renin release, but had no effect on the formation of 6-keto PGF 1α, a stable metabolite of PGI 2; (2) Indomethacin (2×10 −5 M) had no effect on isoproterenol stimulated renin release, but inhibited 6-keto PGF 1α formation; (3) Dibutyryl cyclic AMP (10 −3 M) stimulated both renin release, and 6-keto PGF 1α release. Indomethacin (2×10 −5 M) did not inhibit dibutyryl cyclic AMP-stimulated renin release, but did inhibit the production of 6 keto PGF 1α. These results indicate that the β-adrenoceptor mediated renin release does not depend on the formation of PGI 2, but renin release is dependent on cyclic AMP formation.