Prospects of dissolved albumin as a chiral selector in capillary zone electrophoresis

Abstract

It has been shown using as examples amino acids, mono- and dicarboxylic acids that the enantioselectivity of dissolved albumin may be employed with advantage in capillary zone electrophoresis (CZE) separations. Slow changes in the enantioselectivity of albumin dissolved in weakly alkaline aqueous solutions can be avoided by a short mild heating of the solution. The efficiency of a separation system for a given solute in CZE is at least one order of magnitude higher than that in LC based on bonded albumin. The good chiral separations obtained here and the high efficiency of CZE separation systems confirm that CZE separations based on dissolved albumin as a chiral selector can compete with analogous LC separations. Perfect qualitative agreement of both enantioselective properties of albumin and factors affecting its enantioselectivity in CZE and LC proves that the knowledge obtained in LC with bonded albumin may be transferred to CZE separation systems.