Prospects for the use of modern methods of staining nucleolar organizers regions of cells for the diagnosis of diseases in animals

Abstract

Relevance.In recent years, issues of human and animal oncology have been considered in a broad aspect as a separate interdisciplinary science. Some diseases are more common in animals than in humans. That is why the selection of the necessary methods of purposeful value is required. In connection with the above data, the relevance of the work lies in the consideration of various methods of using NOR cells for the diagnosis of diseases of various etiologies in animals.Methods and results.In order to determine a particular disease, it is necessary not only to carefully study the available histopathological methods, but also to develop new ones. The comparability of human, bovine, sheep, and other mammalian gene maps opens up the possibility of applying methods used in medicine for animals (for example, FISH analysis with human DNA probes). Argyrophilic proteins associated with NOR ofcells are widely used in diagnostic pathology of a different nature. AgNOR staining method is considered as a proliferation marker. The use of the NOR method is promising in the differentiation of malignant and benign diseases. This method consists of counting the number of nuclei, determining the area and size of the nucleoli. Thus, methods based on the calculation of NOR are the most promising for diagnosing various pathologies in animals, including taking into account the prognosis of diseases.