Prospects for the use of layer-by-layer technology for the development of an enzyme biosensor used for milk quality control

Abstract

This article presents the results of scientific research on the development of a biosensor system for the determination of salts of heavy metals (cadmium and lead) in milk. When developing a biosensor system, much attention is paid to the selection of biological material and the method of their stabilization using physical and chemical forces, namely, immobilization. In this regard, studies were carried out on the selection of the enzyme and the method of its immobilization in the development of a biosensor for the detection of salts of heavy metals in milk. The development of a biosensor for the determination of toxic elements in food products is of scientific and practical importance. In world practice, special attention is paid to the contamination of raw materials and food products with toxic chemicals, mainly of anthropogenic origin, which are persistent organic pollutants. Therefore, quality control of raw materials, food products is important for consumers, and, accordingly, for the food industry. Experimental studies are based on the methods of Filippova A. M., Vorobieva O. V. to determine the specific activity of the enzyme acetylcholinesterase. Determination of the activity of the catalase enzyme was carried out in accordance with the gasometric method according to the method of Warburg. As a result of experimental studies, the catalase enzyme as a biological material was chosen as a sensitive element. As a carrier for the immobilization of enzymes, a 5-bilayer combination of "chitosan-sodium alginate" was selected. The layer-by-layer technology was used to immobilize the enzyme on the substrate surface when creating an enzyme biosensor. The results of the research are recommended to be applied in the study of food safety issues and in the assessment of safety indicators of raw materials and food products by the express method.