Abstract
Background. In present days, much attention is paid to the study of the interrelation between the macrophage/hystiocytic microenvironment and the tumor substrate in lymphoproliferative disorders.Objective. The article is devoted to the morphometric and morphological assessment of CD163-positive macrophages in nodular sclerosis Hodgkin lymphoma.Materials and methods. Formalin fixed, paraffin-embedded (FFPE) lymph node samples of 45 patients were used for the study. To identify and visualize CD163-positive cells in the test material, an immunohistochemical staining method was used.Results. The study shows that the morphometric and morphological analysis of CD163-positive cells can be an effective and promising criterion for representing them as potential predictors of the disease course. Immunohistochemical study of 45 cases using the CD163 marker revealed a difference in the nature of macrophages localization in the lymph nodes nodules. The dependence of CD163-expressing cells number on the disease course was determined.Conclusion. The data obtained can be used to stratify patients with nodular sclerosis of classical Hodgkin lymphoma into risk groups and to determine personalized approaches to treatment. Immunohistochemical determination of the CD163 marker can be used in the complex diagnosis of the causes of refractoriness to the first and subsequent lines of therapy.
Highlights
Much attention is paid to the study of the interrelation between the macrophage/hystiocytic microenvironment and the tumor substrate in lymphoproliferative disorders
Compliance with patient rights and principles of bioethics The study protocol was approved by the biomedical ethics committee of Kirov Research Institute of Hematology and Blood Transfusion, Federal Medical Biological Agency
Summary
The article is devoted to the morphometric and morphological assessment of CD163‐positive macrophages in nodular sclerosis Hodgkin lymphoma. Морфометрическая оценка CD163‐положительных макрофагов при кЛХ может выступить эффективным и перспективным методом представления их как по тенциальных предикторов течения заболевания. Актуальным и целесообразным оста ется изучение прогностического значения клеток опу холевого микроокружения при кЛХ. В 1‐ю группу (n = 17) вошли больные, достигшие пол ной ремиссии при проведении стандартной полихи миотерапии 1‐й линии по схеме BEACOPP-14 (условно благоприятное течение заболевания). Во 2‐ю группу (n = 28) включены пациенты с рефрактерностью к хи миотерапии или минимальным ответом на терапию 1‐й и последующих линий, впоследствии получившие ау тологичную или аллогенную трансплантацию гемопо этических стволовых клеток (условно неблагоприятное течение заболевания). Патоморфологическую оценку и подсчет относительного числа CD163‐экспресси рующих макрофагов выполняли на световом микро скопе LeicaDM 1000 (Германия) со встроенной фотои видеокамерой с помощью программного обеспечения анализа изображений ImageScope Color (версия М) с окулярами × 10 при объективах × 4, × 10, × 40, × 100.
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