Prospects for Genetic Improvement in Internal Nitrogen Use Efficiency in Rice

Terry Rose,Tobias Kretzschmar,Matthias Wissuwa,Jeanette Balindong,Daniel L E Waters

doi:10.3390/agronomy7040070

Abstract

While improving the efficiency at which rice plants take up fertiliser nitrogen (N) will be critical for the sustainability of rice (Oryza sativa L.) farming systems in future, improving the grain yield of rice produced per unit of N accumulated in aboveground plant material (agronomic N use efficiency; NUEagron) through breeding may also be a viable means of improving the sustainability of rice cropping. Given that NUEagron (grain yield/total N uptake) is a function of harvest index (HI; grain yield/crop biomass) × crop biomass/total N uptake, and that improving HI is already the target of most breeding programs, and specific improvement in NUEagron can only really be achieved by increasing the crop biomass/N uptake. Since rice crops take up around 80% of total crop N prior to flowering, improving the biomass/N uptake (NUEveg) prior to, or at, flowering may be the best means to improve the NUEagron. Ultimately, however, enhanced NUEagron may come at the expense of grain protein unless the N harvest index increases concurrently. We investigated the relationships between NUEagron, total N uptake, grain yield, grain N concentration (i.e., protein) and N harvest index (NHI) in 16 rice genotypes under optimal N conditions over two seasons to determine if scope exists to improve the NHI and/or grain protein, while maintaining or enhancing NUEagron in rice. Using data from these experiments and from an additional experiment with cv. IR64 under optimum conditions at an experimental farm to establish a benchmark for NUE parameters in high-input, high yielding conditions, we simulated theoretical potential improvements in NUEveg that could be achieved in both low and high-input scenarios by manipulating target NHIs and grain protein levels. Simulations suggested that scope exists to increase grain protein levels in low yielding scenarios with only modest (5–10%) reductions in current NUEagron by increasing the current NHI from 0.6 to 0.8. Furthermore, substantial scope exists to improve NUEveg (and therefore NUEagron) in high-yielding scenarios if maintaining current grain protein levels of 7.3% is not essential.

Highlights

Nitrogen (N) is required in large quantities by crops and N deficiency frequently limits rice grain yields in traditional rice (Oryza sativa L.) farming systems [1]
The present study investigated the relationships between NUEagron, total N uptake, grain yield, grain N concentration and N harvest index (NHI) in 16 rice genotypes under optimal N conditions in order to determine if scope exists to improve the NHI and/or grain protein, while maintaining or enhancing NUEagron in rice
The average grain yield exceeded 5 t ha−1 in the dry season compared to 3.4 t ha−1 in the wet season and the higher yield during the dry season was a result of both greater total biomass yield

Summary

Introduction

Nitrogen (N) is required in large quantities by crops and N deficiency frequently limits rice grain yields in traditional rice (Oryza sativa L.) farming systems [1]. Nitrogen losses from fields result in N fertiliser recoveries of 20–40% where N fertiliser is used in lowland rice production [2]. The majority of these losses are thought to be through NH3 volatilisation and full denitrification of NO3 − to N2 , as a result of the highly reduced soil conditions that occur in flooded rice systems [3]. There is significant interest in improving rice N efficiency through breeding in addition to optimising agronomic management of N fertilisers [1]. Gains could be made by improving either the N acquisition efficiency or internal N utilisation efficiency [1,8]

Methods

Results

Discussion

Conclusion