Prospective open pilot study on the use of cam oleum ointment for feline calicivirus disease in cats in Baghdad City

Abstract

Feline calicivirus (FCV) is a highly contagious virus that causes a mild to severe respiratory infection and oral disease in cats. It is especially common in shelters and breeding colonies, and often infects young cats, this study contained 50 different cats and samples were collected from September 2020 to January 2021. Samples taken by swabs from oropharyngeal and conjunctival area depending on the lesion of FCV infection to investigate viral nucleic acid from collected swabs, then extracting RNA from the swabs and converting it to a cDNA molecule, and last detecting the open reading template gene 2 using specific primer, these samples isolated from veterinary clinics, and shelters, all samples were collected from Baghdad city .Rapid test AG kit and Real time PCR were done to confirm the detection and the results gave us 28 positive results on rapid AG kit and RT-PCR results gave us 32 positive samples and the other 18 samples gave us negative results by detection with nanodrop device before sending all samples for molecular investigation by polymerase chain reaction. Most cats recover completely after a calicivirus infection when we treated with Camoleum ointmentprepared in veterinary medicine college lab.