Prospective Application of Partially Digested Autologous Chondrocyte for Meniscus Tissue Engineering.

Chung-Liang Chien,Ching-Chuan Jiang,Hongsen Chiang,Piya-on Numpaisal,Chang-Hsun Hsieh

doi:10.3390/pharmaceutics14030605

Chung-Liang Chien, Ching-Chuan Jiang + Show 3 more

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https://doi.org/10.3390/pharmaceutics14030605

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Abstract

Background: Meniscus tissue engineering has yet to achieve clinical application because it requires chondrogenic induction and in vitro cell expansion. Contrarily, cartilage engineering from autologous chondrocytes has been successfully applied in one-stage surgery. If the natural chondrogenic potential of meniscus cells can be demonstrated, meniscus tissue engineering would have more value in clinical settings. Materials and Methods: In total, 10 menisci and pieces of cartilage were obtained during total knee replacements. The tissues were collected for cell isolation and expansion. Their chondrogenic properties were examined by immunohistofluorescence and gene expression analyses. Results: In native cartilage, immunofluorescence demonstrated the presence of collagen I, aggrecan, and traces of collagen I, whereas comparable staining was seen in the inner and middle meniscus. The presence of collagen I but the absence of collagen II and aggrecan were observed in the outer meniscus. In passage 2, chondrocytes showed the presence of collagen II and aggrecan, and the absence of vimentin. The vimentin and aggrecan staining were comparable in the inner and middle meniscus cells, whereas the outer cells showed only vimentin staining. In the gene expression analyses, the expressions of collagen II and aggrecan in the native chondrocyte and the inner and middle meniscus were higher than those of the cells from the outer meniscus, but they were not different in collagen I. In the passage 2 culture, chondrocytes had a higher expression of collagen II and aggrecan than the meniscus cells. Cells from the inner and middle areas had higher collagen II and aggrecan expression than those from the outer meniscus. Conclusion: Without chondrogenic induction, inner and middle meniscus cells possess a chondrogenic phenotype. Specifically, native meniscus cells exhibited more robust chondrogenic potential compared with those of the passage 2 monolayer culture.

Highlights

In the staining of the middle and outer meniscus, the majority of collagen I and collagen II had fibrillar-like patterns
The co-localization of collagen I and II was observed in the middle and outer meniscus, but the middle meniscus had predominant collagen II staining whereas the outer meniscus presented more staining of collagen type I than collagen type
The immunofluorescence of the native meniscus resembled that of previous studies [22,23]: the inner meniscus carried more collagen type II and aggrecan than collagen type I; the middle transitional zone showed a comparable expression of collagen type II, aggrecan, and collagen type I; the outer meniscus showed predominantly the presence of collagen type I

Summary

Introduction

The fibrocartilagenous meniscus of the knee functions to protect articular cartilage for both load transmission and shock absorption across the joint [1,2]. For this reason, the meniscus is vulnerable to injuries. Results: In native cartilage, immunofluorescence demonstrated the presence of collagen I, aggrecan, and traces of collagen I, whereas comparable staining was seen in the inner and middle meniscus. In the gene expression analyses, the expressions of collagen II and aggrecan in the native chondrocyte and the inner and middle meniscus were higher than those of the cells from the outer meniscus, but they were not different in collagen I. In the passage 2 culture, chondrocytes had a higher expression of collagen

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