Abstract
The current study prospects the antimicrobial potential of an endophytic fungus Chaetomium globosum which showed a wide spectrum antimicrobial activity against the tested pathogenic microorganisms. This is apparently the first report where Chaetomium globosum as an endophyte from Moringa oleifera showed antimicrobial potential and is optimized for physiochemical parameters to enhance the antimicrobial metabolites production. In the classical optimization yeast peptone dextrose medium, inoculum size of two discs, incubation period of 6 days, production temperature of 25 ºC and pH 7 was best supportive for optimal growth and antimicrobial activity whereas maltose and ammonium nitrate were the best carbon and nitrogen sources, respectively. The statistical optimization resulted in up to 1.33 fold increase in antimicrobial activity. Chloroform was found to be the best extractant. The chloroformic extract showed minimum inhibitory concentration ranging from 0.05 to 5 mg/ml and its microbicidal nature was established by viable cell count studies. The efficacy of the extract was also established in terms of post antibiotic effect which ranged from 2 to 20 h. The chloroformic extract exhibited the good antibiofilm potential and was also found to be biosafe. The clinical relevance of the study was justified as it showed good antimicrobial efficacy against some resistant clinical isolates, too.
Highlights
The emergence and spread of antimicrobial resistance by pathogenic microorganisms to commercial drugs is a noteworthy issue endured by mankind and has turned into an important concern throughout the globe (Rajeswari et al 2016)
The burning problem of increasing microbial resistance has led the researchers to look for the novel sources of antimicrobial agents to control the drug resistant pathogens
An endophytic fungal isolate C. globosum, obtained from Moringa oleifera has been studied for its antimicrobial potential
Summary
The emergence and spread of antimicrobial resistance by pathogenic microorganisms to commercial drugs is a noteworthy issue endured by mankind and has turned into an important concern throughout the globe (Rajeswari et al 2016). The human health has been much dependent on plants since ages, because of the wide spread belief that ‘green medicines’ are healthier and safer than the synthetic ones (Newman and Cragg 2007) Another reason for the consideration of natural resources in the field of healthcare is the increasing incidences of multidrug resistant organisms arising from the consistent use of synthetic medicines for the treatment of various ailments (Selvamohan et al 2012). Microbial species present in interand intracellular spaces of plant tissues without causing
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