Abstract
A Gram-stain-negative, non-spore-forming, non-motile, short-rod-shaped, and aerobic bacterial strain (designated L72T) was isolated from propylene oxide saponification wastewater activated sludge obtained from a wastewater treatment facility in Binzhou (Shandong Province, PR China). Strain L72Tgrew between 25 and 40°C (optimum growth at 30°C). The pH range for growth was between 6.0 and 8.0 (optimum growth at pH 7.0). The range of NaCl concentrations for the growth of strain L72Twas 0-3.0 % (w/v), with optimum growth at 1.0-2.0 % (w/v). The major cellular fatty acids of strain L72Twere C19:0cyclo ω8c, C18:1ω7c,iso-C15:0, and anteiso-C15:0. Strain L72Tcontained Q-10 as the predominant respiratory quinone. The polar lipid profile was composed of Phosphatidylcholine, Glycolipid, Aminophospholipid, Phosphatidylethanolamine, Phosphatidylserine, Phosphatidyldimethylethanolamine, one unknown lipid (L) and two unidentified Phospholipids (PL). Genome sequencing revealed a genome size of 4,703,686bp and a G + C content of 69.0mol%. The 16S rRNA gene sequence similarities of strain L72Twith other species were less than 94%. Phylogenetic analyses based on 16S rRNA gene sequences and genome data, revealed that strain L72Tformed a distinct phylogenetic lineage within the order Hyphomicrobiales, separating them from members of all families. Strain L72Tshowed 70.7% average nucleotide identity and 18.6% digital DNA-DNA hybridization identity with the closely related speciesRhodoligotrophos defluvii. Based on the phenotypic, phylogenetic and chemotaxonomic data, a new familyPropylenellaceaefam. nov. comprising the genusPropylenellagen. nov. and speciesPropylenellabinzhouensesp. nov. is proposed. The type strain is L72T(= CCTCC AB 2019081T = KCTC 72254T).
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