Abstract
Abstract Single intraperitoneal injections (of 0.5, 1.0, and 2.0 ml per kilogram body weight) of a solution of sodium pentobarbital (50 mg/ml, in propylene glycol, 40 ml/100 ml) elevated rat serum triglyceride values obtained by the semi-automated fluorometric method of Kessler and Lederer. Increases of 267, 619, and 813%, respectively, were noted within 5 min. Liver triglycerides and other major lipid fractions in serum and liver were unaffected. The solution added directly to pooled rat sera in vitro also elevated the apparent triglyceride concentrations, propylene glycol being the responsible agent. Essentially all of the propylene glycol was recovered as apparent triglyceride. This type of chemically induced artifact can be corrected for by determining a blank on each unknown (i.e., the hydrolysis step is omitted from the procedure). Subtraction of the blank concentration from the total triglyceride concentration yields the true triglyceride concentration. Extraction of the unknown by the Folch procedure also eliminates interference from propylene glycol.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
