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Abstract
Antibody can be assessed by agglutinating antibody titer which is a quantitative measure of circulating antibodies in serum from fish previously immunized. The antibody evaluation has been performed with different fish species, and is considered a reliable method that can be applied to confirm several hypothesis regarding acquired immunity, even in conjunction with precise methods to describe immune mechanisms. In order to provide appropriate analytical methods for future studies on the specific immune system of native fish, the present study standardized on assay to measure the serum agglutinating antibody titer produced after immunization with inactivated A. hydrophila and levamisole administration in pacu. It was possible to determine the agglutinating antibodies titer in a satisfactorily way in pacu immunized with inactive A. hydrophila, and the highest titers were observed on fish fed with levamisole.
Highlights
Fish are the most primitive vertebrate group to present acquired immune system, and the ability to produce antibody after antigenic stimuli
Antibodies are glycoproteins classified as immunoglobulins (Ig) that can be expressed in the B lymphocyte membrane or be released by plasma cells in several body fluids (Solem and Stenvik, 2006)
After this period fish from control and levamisole fed groups were immunized with inactive A. hydrophila and remained in their aquarium up to 15 days
Summary
Fish are the most primitive vertebrate group to present acquired immune system, and the ability to produce antibody after antigenic stimuli. Antibodies are glycoproteins classified as immunoglobulins (Ig) that can be expressed in the B lymphocyte membrane or be released by plasma cells (activated B lymphocytes post-connect with antigen) in several body fluids (Solem and Stenvik, 2006). Most of mammals have five classes of immunoglobulins: IgG, IgM, IgA, IgD and IgE, differing in structure and biological activities. The development of effective measures to confer a better health status to fishes, such as the improvement of antibacterial or antiviral vaccines or by diet immune modulation can be useful for fish aquaculture (Muiswinkel, 2008). The development of these strategies depends on a better understanding of
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