Proposed Design Model of Single use Bioreactor for Mesenchymal Stem Cells Proliferation

Abstract

Bioreactor, the womb for human stem cells, plays a vital role in enhancing its proliferation under controlled environment. Stem cells have the potential to differentiate and replicate it-self to further grow human tissues and organs. Bioreactor nurtures stem cells by providing nutritious environment and peerless oxygen flow inside the cell bag without obstructing the sheer force of the fluid or gas bubbles. The present paper unveils the design and development of bioreactor using the gyrator motion mechanism. This high functional bioreactor provides automation system that enhances the environment and serves excellent nutrient distribution to exceed cell growth through oxygen transfer and better mixing. One of the best characteristic of this bioreactor is that it requires no cleaning or sterilization as it is disposable. Compared to other bioreactors such as spinner flask type, rotating-wall type and the hollow fibre type, the proposed bioreactor is more convenient, easy to operate and maintain with more flexibility. Proposed bioreactor system operates in conjunction with mechanism of external gyrating motion that uses gravity in a natural way to make the flow circular inside it. The simultaneous mixing motion base is extremely suitable to grow stem cells. This bioreactor can be easily placed in an incubator and further addition or sampling is viable without the use of laminar flow cabinet.