Abstract
Readouts from the thrombin generation (TG) assay (TGA) have been used to predict the effects of hemostatic drugs in hemophilic patients (1). There is better correlation between individual's bleeding tendency and their TG capacity compared to factor levels measured using routine assays (2-3). However, the clinical utility of TGA has not yet been validated in multicenter studies because of lack of standardization. This report aims to highlight variables that may impact directly on TGA reliability, and steps to reduce TGA variability within and between laboratories to improve precision of results. There are two semi-automated (CAT (Stago, Asnieres, France), Technothrombin-TGA (Technoclone, Viena, Austria) and two fully-automated (CEVERON® alpha w. TGA (Technoclone), ST-Genesia (Stago)) instruments that use fluorogenic thrombin substrates. Three instruments on the market use chromogenic thrombin substrates (HemoScan Thrombin Generation Assay (HemoScan, Groningen, The Netherlands), Pefakit in-TDT (Pentapharm, Aesch, Switzerland), Innovance ETP (Siemens Healthcare SAS, Erlangen, Deutschland). This article is protected by copyright. All rights reserved.
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