Abstract
Over the last few years, propolis has been the object of many studies conducted around the world, and its biological properties and chemical composition have been widely investigated. The present study focuses on the evaluation of the antimicrobial activity as well as an examination of the chemical composition of two samples of propolis from Eastern Algeria coming from the commune of El Mechrouha and Ouled Driss in the wilaya of Souk-Ahras. The two samples are tested for their antimicrobial power by undertaking the agar diffusion technique on eight pathogenic microbial strains (six bacterial strains and two fungal strains) which are: Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus thoraltensis, , , Candida famata and Aspergillus niger. The results obtained clearly show the impact of propolis on the microbial susceptibility of Gram-positive bacteria (S. agalactiae and S. aureus), as well as on fungal species (C. famata and A. niger). The analysis of the chemical composition of the ethanolic extracts of the two propolis by UV-visible absorption spectrometry and thin layer chromatography showed that Algerian propolis is wealthy in phenolic compounds, and high performance liquid chromatography allowed the identification of four polyphenols (Gallic acid, Caffeic acid, Quercetin and Catechin). These outcomes permitted a first assessment of the two propolis which present comparable components in their chemical compositions.
Highlights
Apitherapy is one of the practices in natural care, based on products of the hive such as: honey, royal jelly, propolis ... etc
The antimicrobial activity of the extracts was estimated regarding the diameter of the inhibition zone around the discs containing the extracts to be tested against pathogenic microorganisms which are: S. aureus, S. agalactiae, S. thoraltensis, K. pneumonia, E. coli, P. aeruginosa, C. famata and A. niger
Our work, which is devoted to the study of the antimicrobial property of two samples of propolis from Eastern Algeria, has enabled us to observe that the field of beehive products is a vast and fascinating field of scientific research
Summary
Apitherapy is one of the practices in natural care, based on products of the hive such as: honey, royal jelly, propolis ... etc. Propolis of temperate areas is essentially composed of phenolics such as flavonoids and cinnamic acid byproducts. The antimicrobial activity and chemical properties of propolis are essential parameters for its characterization (Silici et al, 2007). All these data led us to study the biological properties of two propolis from Eastern Algeria. A comparative study based on antimicrobial activity was carried out: the antibacterial and antifungal effects of propolis extracts were tested on clinically pathogenic microorganisms including bacteria, fungus and yeast. Our work focused on polyphenols and more flavonoids, which are essential elements of several biological activities of propolis, like the antimicrobial one. The identification and structure determination of these components is carried out by visible UV spectrometry, as well as other chromatographic techniques
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