Abstract
Hepatocellular carcinoma (HCC) is the leading cause of tumor death in China with high mortality since its strong metastatic potential. Currently, treatment against advanced HCC is poorly efficient and thus screening new drugs to prevent the HCC invasion is of great significance to improve the survival rate of patients with HCC. From the results of this study, we concluded that propofol, a widely used anesthetics could prevent the proliferation by MTT assay. The scratch wound and invasion assays showed that migratory property and invasiveness in HCC cells SMMC-7721 was inhibited by propofol. This process was probably mediated by NET1 since NET1 overexpression offset the repressive effect of propofol on the invasiveness and migratory ability of SMMC-7721 cells. Furthermore, propofol treatment also reduced p-ERK1/2 and VEGF level by western blot analysis. Similar observation was found when NET1 was silenced. Thus, the results of this study provided valuable clinical therapy potential of propofol against liver cancer. We also disclosed molecular mechanism underlying the regulation of invasion and migration in HCC cells by NET1.
Highlights
Liver cancer is a fatal cancer and presented the second mortality rate in the w orld[1,2]
We found that NET1 level in hepatic cancer cell line including Hep-G2, Huh[7], SMMC-7721 and HL-7702 was significantly higher than that in normal HEK293T cells (Fig. 1C,D)
We found NET1 regulated propofol-induced inhibitory effect on liver cancer cell invasion and migration
Summary
Liver cancer is a fatal cancer and presented the second mortality rate in the w orld[1,2]. Hepatocellular carcinoma (HCC) belonging to primary liver cancer[3] acts as the third leading mortality of tumor‐related deaths in China[4]. In HCC, propofol can inhibit proliferation, migration and invasion of liver cancer cells[14]. Propofol inhibited tumor progression of hepatocellular carcinoma xenografts in BALB/C mice[15]. Propofol induces apoptosis of hepatocellular carcinoma c ells[16]. All these studies indicated that propofol maybe a candidate drug for liver cancer. We investigated the role of propofol in HCC by regulating the NET1 expression. We indicated the inhibitory effect of propofol on HCC cell invasion and migration mediated by NET1. This work vali‐ dated the potential value of propofol in the treatment of liver cancer
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