Propofol inhibits eryptosis of human erythrocyte induced by hydrogen peroxide in vitro

Abstract

Objective To explore the effects of propofol on eryptosis stimulated by hydrogen peroxide (H2O2),which was analyzed by phosphatidylserine (PS) exposure at erythrocyte surface and forward scatter (FSC) of erythrocyte in vitro. Methods Human erythrocytes isolated from healthy volunteers were made to 2％ suspension and divided into 5 groups:group C (ringer solution alone),group H (H2O2 alone),group P10+H ( 10 μmol/L of propofol and H2O2),group P50+H (50 μmol/L of propofol and H2O2)and group P100+H (100 μmol/L of propofol and H2O2).At the same time,Ionomycin,Vitamin E and Intralipid were used to establish positive and negative control.H2O2 was used at a concentration of 200 μmol/L.Both erythrocyte PS exposure and FSC were determined by flow cytometry after 1 hour.Data were analyzed in One-Way ANOVA by SPSS. Results Compared to group C,H2O2 at 200 μmol/L significantly increased the PS exposure of erythrocyte (15.20±1.01) vs (1.45±0.21)(P＜0.001 ) and decreased the FSC (1 768±9) vs( 1 808±26)(P＜0.05),which were effectively improved by propofol at 50 μmol/L (15.20±1.01) vs(3.09±1.66) in PS exposure(P＜0.001 ),(1 768±9) vs (1 811±16) in FSC(P＜0.05). Conclusions H2O,induces human erythrocyte eryptosis in vitro,which can be effectively attenuated by propofol because of it's strong antioxidant and free radical scavenging activity. Key words: Erythrocyte; Phosphatidylserine; Propofol; Hydrogen peroxide