Propionibacterium freudenreichii CIRM-BIA 129 Osmoadaptation Coupled to Acid-Adaptation Increases Its Viability During Freeze-Drying.

Floriane Gaucher,Philippe Blanc,Romain Jeantet,Gwénaël Jan,Koffigan Kponouglo,Julien Jardin,Pierre Marchand,Valérie Briard-Bion,Sylvie Bonnassie,Sandrine Pottier,Jordane Ossemond,Houem Rabah

doi:10.3389/fmicb.2019.02324

Abstract

Propionibacterium freudenreichii is a beneficial bacterium with documented effects on the gut microbiota and on inflammation. Its presence within the animal and human intestinal microbiota was correlated with immunomodulatory effects, mediated by both propionibacterial surface components and by secreted metabolites. It is widely implemented, both in the manufacture of fermented dairy products such as Swiss-type cheeses, and in the production of probiotic food complements, under the form of freeze-dried powders. The bottleneck of this drying process consists in the limited survival of bacteria during drying and storage. Protective pre-treatments have been applied to other bacteria and may, in a strain-dependent manner, confer enhanced resistance. However, very little information was yet published on P. freudenreichii adaptation to freeze-drying. In this report, an immunomodulatory strain of this probiotic bacterium was cultured under hyperosmotic constraint in order to trigger osmoadaptation. This adaptation was then combined with acid or thermal pre-treatment. Such combination led to accumulation of key stress proteins, of intracellular compatible solute glycine betaine, to modulation of the propionibacterial membrane composition, and to enhanced survival upon freeze-drying. This work opens new perspectives for efficient production of live and active probiotic propionibacteria.

Highlights

Incidence of inflammatory bowel disease (IBD), of irritable bowel syndrome (IBS) and of antibioticassociated diarrhea (ADD) is increasing in developed countries in relation with modifications of the life style
We investigate the impact of the modulation of the fatty acid composition, compatible solutes accumulation and expression of general stress proteins, on P. freudenreichii tolerance toward different stresses and during freeze-drying
P. freudenreichii CIRM-BIA 129 viability was determined by numeration (CFU counting) before and after these challenges, FIGURE 1 | Viability of Propionibacterium freudenreichii CIRM-BIA 129 after freeze-drying (A), heat (B), acid (C), and bile salts (D) challenges

Summary

Introduction

Incidence of inflammatory bowel disease (IBD), of irritable bowel syndrome (IBS) and of antibioticassociated diarrhea (ADD) is increasing in developed countries in relation with modifications of the life style. The diet has an important impact on the modulation of the microbiota. A dysbiosis of the microbiota is involved in many disorders like inflammation, allergy and atopy (Moco et al, 2014; Skonieczna-Zydecka et al, 2018; Soularue et al, 2018). Ingestion of a mixture of probiotic bacteria induced or Propionibacteria Freeze-Drying Adaptation prolonged a remission in patients suffering from ulcerative colitis (Ganji-Arjenaki and Rafieian-Kopaei, 2018). Exposition to key immunomodulatory symbiotic bacteria and or/probiotic prevent allergy and atopy (Fujimura et al, 2016; Butel et al, 2018). Allergic diseases in Caesarean-delivered children, at 13-year follow-up, was prevented by an early exposition to a mixture of lactic acid bacteria an propionibacteria (Kallio et al, 2018)

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