Abstract
The characteristics of Escherichia coli E-26 and a derived mutant E-26V during adaptation to propionate have been examined. When transferred from a glucose citrate medium to a mineral salts medium containing propiouate as the sole source of carbon, E-26V initiated growth more quickly and formed a higher activity of α-hydroxyglutarate synthase than did the parent strain. Moreover, in E-26V, the formation of α-hydroxyglutarate synthase immediately preceded growth on propionate, whereas in E-26, enzyme activity was not detected until the mid to late exponential growth phase. In contrast, isocitrate lyase formation immediately preceded growth initiation in both the parent and mutant cultures. It is proposed that the α-hydroxyglutarate pathway is metabolically significant during growth of E. coli E-26V on propionate by providing an auxiliary mechanism for the formation of C 4 acids. Such intermediates are essential for the formation of adaptive enzymes involved in the oxidation of propionate to acetate.
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