Propil Tiyourasil (Ptu) Ve L-Tiroksin İle Oluşturulan Deneysel Hipo- Ve Hipertiroidizmde Erişkin Sıçanların Hipokampüsünde Doku Lipid Peroksidasyonu, Glutatiyon Ve Antioksidan Enzim Düzeyleri üzerine araştırmalar

Abstract

Background: Thyroid hormones (TH) thyroxine (T4) and triiodothyronine (T3) are known to be essential for maintaining optimal cognitive ability in adults. TH deprivation (hypothyroidism) or excess (hyperthyroidism) may alter signaling pathways involved in adult hippocampal neurogenesis and synaptic plasticity, thus increasing the risk of neurodegenerative disorders and leading to changes in cognitive performance. Aim: This study aimed to explore the effect of experimental hypo- and hyperthyroidism on the lipid peroxidation and glutathione levels, and the activities of antioxidant system enzymes SOD, catalase (CAT) and glutathione peroxidase (GPx) and glutathione reductase (GR) in the rat hippocampus. Material and Methods: The study included 72 adult male Wistar albino rats which were grouped as follows: (1) control; (2) hypothyroidism [hypothyroidism was induced by intraperitoneal injection of 10 mg/kg/day propylthiouracil (PTU) for 4 weeks]; (3) hyperthyroidism [hyperthyroidism was induced by 4-weeks' thyroxine injection (0.3 mg/kg/day)]. The levels of lipid peroxidation, glutathione, antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) in the hippocampus of rats with hypo- and hyperthyroidism were examined using ELISA techniques. Results: The ELISA analysis results of our study show that in the hyperthyroid and hypothyroid rats, the lipid peroksidasyon levels in the hippocampus did not differ significantly from the control groups (P