Prophylactic Effect of Lactobacillus plantarum YS4 on Oxazolone-Induced Colitis in BALB/c Mice.

Ruokun Yi,Fang Tan,Xin Zhao,Wenfeng Li,Jianfei Mu,Huayi Suo,Xianrong Zhou,Peng Xie

doi:10.1155/2020/9048971

Abstract

In the present research, the effects of Lactobacillus plantarum YS4 (LP-YS4) on colitis were tested in an oxazolone-induced mouse model. BALB/c mice were induced by oxazolone and then treated with LP-YS4. The serum levels of mice were analyzed using commercial kits and the protein and mRNA expression levels of mouse colon tissue were detected by Western blotting and qPCR assay, respectively. The results demonstrated that LP-YS4 significantly (P < 0.05) increased the colon length and ratio of colon weight/length in mice with colitis and attenuated the negative effects of colitis. The results also showed that treatment with LP-YS4 significantly reduced the serum concentrations of ET-1, SP, and IL-10 while significantly increasing those of SS, VIP, and IL-2 in colitis mice (P < 0.05). In addition, LP-YS4 significantly increased the activities of GSH and SOD while decreasing those of MPO and MDA in the colon tissue of colitis mice (P < 0.05). LP-YS4 also significantly upregulated the mRNA and protein expression of c-Kit, eNOS, nNOSe, and SCF in colitis mice while significantly downregulating the relative expression of iNOS. In summary, LP-YS4 could reduce the negative effects of colitis, and such effects were better than those of the common probiotic Lactobacillus bulgaricus.

Highlights

Ulcerative colitis is a type of inflammatory bowel disease that can potentially lead to cancer. e age of onset of colitis is typically 20–50 years old, and it can seriously threaten the quality of life of patients [1]. e immunopathogenesis and immunosuppressive treatment of colitis are currently the research topics of significant interest. e research goals are to diagnose and treat colitis in order to prevent exacerbation of the disease [2]. e drugs used to treat colitis in the clinic often have adverse effects after their long-term application
The potential effects of Lactobacillus plantarum YS4 (LP-YS4) on oxazolidone-induced colitis were investigated for the first time. e findings provide a possible foundation for further development of LP-YS4, especially its application in functional food or medicine
LLC, USA; IL-2, IL-10, ET-1, Substance P (SP), SS, and Vasoactive Intestinal Peptide (VIP) serum cytokine kits were purchased from BioLegend Inc., USA; GSH, Superoxide Dismutase (SOD), MPO, and MDA kits were purchased from Nanjing Jiancheng Bioengineering Institute, Nanjing, China; Trizol reagent, oligodT18, RNase, dNTP, MLV, primer, BCA protein quantitative kit, APS, TEMED, SDS-PAGE, PVDF membrane, first antibody, and second antibody were purchased from ermo Fisher Scientific, Inc., USA

Summary

Introduction

Ulcerative colitis is a type of inflammatory bowel disease that can potentially lead to cancer. e age of onset of colitis is typically 20–50 years old, and it can seriously threaten the quality of life of patients [1]. e immunopathogenesis and immunosuppressive treatment of colitis are currently the research topics of significant interest. e research goals are to diagnose and treat colitis in order to prevent exacerbation of the disease [2]. e drugs used to treat colitis in the clinic often have adverse effects after their long-term application. E drugs used to treat colitis in the clinic often have adverse effects after their long-term application. Another crucial area of colitis research is focused on the discovery of functional foods that can prevent colitis without side effects [3]. Yak yoghurt is a natural fermented food that is rich in nutrients and is common in the minority areas of the Qinghai Tibet Plateau. Previous research has suggested that yak yoghurt exerts various physiological activities such as antioxidation, cholesterol reduction, and immunity. A prior study on the intestinal physiological activity of lactic acid bacterial species in Yak yoghurt showed that the lactic acid-producing bacteria isolated from yak yoghurt had antioxidant and constipation preventing effects [9]. The potential effects of Lactobacillus plantarum YS4 (LP-YS4) on oxazolidone-induced colitis were investigated for the first time. e findings provide a possible foundation for further development of LP-YS4, especially its application in functional food or medicine

Materials and Methods

Results

Conclusion