Abstract
Dental pulp is the soft live tissue inside a tooth. Dental pulp contains stem cells, known as Dental Pulp Stem Cells. The finest Dental Pulp Stem Cells are found in a baby teeth or milk teeth. The stem cells from the milk teeth are ‘mesenchymal’ type of cells. cells that have the ability to generate a wide variety of cell types like chondrocytes, osteoblasts and adipocytes. To isolate high-quality human dental pulp stem cells from accessible resources is an important goal for stem-cell research. In this study we found that exfoliated human deciduous tooth contains multipotent stem cells, they identified to be a population of highly proliferative, clonogenic cells. Hematopoietic stem cells (HSCs) are the blood cells that give rise to all the other blood cells. HSCs are found in the bone marrow of adults, with large quantities in the pelvis, femur, and they are also found in umbilical cord blood and, in small numbers, in blood. In this study we differentiated stem cells that isolated from human dental pulp and peripheral blood to cardiac cells. The isolation process for dental pulp stem cells would take place in 2 different methods: The enzymatic digestion method and, the second method is a subsidiary one. For isolating the stem cells from peripheral blood we use ficoll method. Flow cytometery analyzes showed that dental pulp stem cells are mesanchymal and peripheral blood cells are hematopoietic stem cells. Bothe group of stem cells can differentiation to cardiac cells. Dental pulp stem cells are mesanchymal cells and peripheral blood cells are hematopoietic cells. For differentiation the cells to cardiomyocite we used 5-azacitidin and exitocin. After differentiation stem cells to cardiac cells, dental pulp stem cells and peripheral blood stem cells morphology was changed, before differentiation to cardiac cells, both group were elongated and after differentiated to cardiomyocit they have central typical nuclear and were stratified ,they expanded cytoplasmic papillae .
Highlights
Among different source for stem cells, less attention has been paid to the human dental pulp
Stem cells in the isolated mononucleotide cells were characterized using a multidisciplinary approach which was based on the expression of stem cell markers, morphology and the capacity to self-renew or proliferate and differentiate into specialized cells. mononucleated cells in suspension were positive for Hematopoietic stem cells (HSCs) markers
mesenchymal stem cells (MSCs) are generally defined as self-renewable, multipotent progenitor cells with the ability to differentiate into several mesenchymal lineages including bone, cartilage, adipose and muscle tissues
Summary
In this study we tried to isolated stem cells from human primary dental pulp and peripheral blood. Peripheral blood mononucleated cells consist of hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs). To date there is no well-defined isolation or characterisation protocol of stem cells from human adult peripheral blood mononucleated cells. Our aim in this study was to isolate and characterize mononucleated cells from human peripheral blood. Peripheral blood mononucleated cells were isolated using the Ficoll-Paque density gradient separation method and cultured in complete medium We want to show that both group of stem cells are the best Candida to clinical treatment, dental pulp stem cells can differentiated to all kind of cells like cardiac cells and are used to treatment disease
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