Abstract
Field samples of banana plants affected by bunchy top disease from Hawaii, Indonesia and Tonga, but not unaffected controls, gave strong positive reactions when tested by enzyme-linked immunosorbent assay using Australian polyclonal and Australian and Taiwanese monoclonal antibodies specific for banana bunchy top virus (BBTV). Isometric virus-like particles, 18-19 nm in diameter, were isolated from these field samples. BBTV-Indonesia, BBTV-Hawaii and BBTV-Tonga all contained a coat protein of Mr about 20 000 and DNA of about 1 kb. The latter two virus isolates had a buoyant density of about 1.30g/cm3 in Cs2SO4. These properties are consistent with those previously described for BBTV-Australia.
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