Abstract
Gene 5 protein of bacteriophage fd was purified from phage infected bacteria. The protein binds to single-stranded DNA but not to double-stranded DNA. The protein inhibits the bacterial exonucleases and, at high concentrations, the cellular DNA polymerases I and II. Gene 5 protein, at low concentrations, increases the template activity of a viral replicative form DNA for polymerase II.
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