Abstract
The hydrogenase system which catalyzes the oxyhydrogen reaction in soybean nodules produced by strains of Rhizobium japonicum is located in the bacteroids. The hydrogenase complex in intact bacteroids has an apparent K m for H 2 of 2.8 μM and an apparent K m for O 2 of 1.3 μM. The addition of hydrogen to bacteroids increases oxygen uptake but decreases respiratory CO 2 production, indicating a conservation of endogenous substrates. After correction for the effect of hydrogen on endogenous respiration a ratio of 1.9 ± 0.1 for H 2 to O 2 uptake was determined. Bacteroids from greenhouse or field-grown soybeans that evolved hydrogen showed no measurable oxyhydrogen reaction activity whereas consistent activity was demonstrated by bacteroids from soybean nodules that evolved little or no H 2 .
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