Abstract
By means of the method of stimulus substitution, single ganglion cells of the isolated perfused retina of Rana esculenta were investigated. The scotopic system was eliminated (a) by a permanent background illumination, (b) by bleaching more than 90 per cent of rhodopsin. The non-appearance of the impulse response during and after light substitution was taken as a criterion for an invariant spectral stimulus-response behaviour (univariance). Only some of the ganglion cells investigated showed this property. The spectral sensitivity of the other cells could be described only by several disconnected branches of functions. Therefore, it was concluded that there exist at least two photopic (or mesopic) mechanisms in the frog retina.
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