Abstract

Dimethyl-methylenedioxy- p-benzoquinone is found to be an excellent mediator for photoreductions by photosystem II only, i.e. in the presence of dibromothymoquinone (DBMIB) blocking reduction by photosystem I. The new quinone system is highly autoxidizable, thus also supporting pseudocyclic electron flow involving photosystem II only. It is not affected by the uncoupler gramicidin at external pH 8, whereas a phenylenediamine acceptor system is strongly inhibited at this pH. This is taken as further support for the notion that the internal pH affects acceptor systems for photosystem II and that therefore the reduction site of these systems occurs in the internal pH range.

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