Abstract
Although the presence of nonspecific inhibitors precluded the use of untreated sera in hemagglutination-inhibition (HI) tests with mouse leukemia virus (MuLV) hemagglutinating antigens, preparations of immunoglobulin G (IgG) lacked nonspecific inhibitory activity and were suitable for use. Hemagglutination (HA) induced by intact, enzyme-treated MuLV and by hemagglutinating MuLV subunits was inhibited by IgG isolated from MuLV antisera. Low titer HI reactions were obtained with IgG from feline leukemia virus (FeLV) antisera. IgG from a broad spectrum of control antisera was noninhibitory. The HI activities of three FMR (Friend-Moloney-Rauscher) antisera against FMR antigens were totally type-specific as revealed by absorption tests. Gross leukemia virus (GLV) antisera also appeared to react type-specifically against GLV antigen. However, the inhibition of FMR antigens by IgG from one nonneutralizing FMR (gs) antiserum, GLV antisera and FeLV antisera, all of which lacked FMR type-specific antibodies, indicated that gs-like reactivity also could be detected by the HI test in at least certain sera. In related studies, an indirect HA reaction employing heterotypic Friend virus antiserum (IgG) revealed a new, possibly monovalent hemagglutinin from degraded GLV. The HA activity of this GLV antigen could be reconstituted by most FMR sera tested, but not by sera directed against GLV, FeLV or other control antigens. The reconstituted hemagglutinin was specifically inhibited by homotypic GLV antiserum (IgG). This indirect HA test not only constitutes direct evidence for the existence of gs antigenic determinants on the surface of the virion but also provides a tool for the detection of such antigens and their corresponding antibodies.
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