Abstract
Abstract Intracytoplasmic membrane vesicles (chromatophores) isolated from Rhodopseudomonas capsulata cells were fused with liposomes by a pH transition procedure. Vesicles of lower density and higher lipid contents and larger diameter than chromatophores were obtained. Similar results were observed by Ca2+ induced fusion and by the freeze-thawing method. Respiratory and light-induced electron transport were measured in chromatophores and fused vesicles. Light-induced reaction center bleaching was observed in all types of vesicles, whereas repiratory electron transport was substantially diminished by lipid incorporation. Ubiquinone 10 restored to some extent respiratory electron transport and oxidative phosphorylation and it modified the photophosphorylation kinetics under continuous light. Electrochromic carotenoid band-shift and the 9-aminoacridine fluorescence quenching indicate that the capacity of the fused vesicles to maintain an electrochemical proton gradient has not been substantially diminished. From the kinetics of 9-aminoacridine quenching an increased K+-permeability seems to be apparent.
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