Properties of melanin from Lachnum YM156 and its hepatoprotective effect in Cd-exposure mice

Abstract

Natural melanin was extracted from Lachnum YM156 (LIM). LIM had better thermostability and light resistance, and its solubility was relatively high under alkaline conditions. Simultaneously, we examined its hepatoprotective effect in Cd-exposure mice. Cd-exposure resulted in decreasing weight growth rate, raised liver index, elevated serum levels of alanine aminotransferase (ALT) and aspartate aminotransaminase (AST), and increased Cd accumulation in livers. Hepatic oxidative stress was evidenced by increased malondialdehyde (MDA) contents, decreased glutathione (GSH) contents, and decreased activities of dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) after CdCl2 administration. Additionally, Cd-exposure also increased the liver level of tumor necrosis factor (TNF)-α and interleukin(IL)-1β. Furthermore, the result of quantitative real-time polymerase chain reaction(qRT-PCR) showed that Cd-exposure reduced the mRNA expression level of NF-E2-related factor 2 (Nrf2), heme oxygenase (HO-1), NADPH quinone oxidoreductase 1 (NQO-1), and elevated the mRNA expression level of factor-kappaB (NF-κB) p65, inducible nitric oxide synthase (iNOS) in livers. However, all these changes were dose-dependently reversed by LIM. Overall, the present findings demonstrated that LIM have the hepatoprotective effect through antioxidant and anti-inflammatory responses in Cd-exposure mice.