Abstract

The mitochondrial genome of Lupinus albus contains 1.2-kb (K1) and 1.3-kb (K2) minicircular DNAs. These minicircles are present in different cultivars of Lupinus albus and in other species which are in close relation to Lupinus albus. The entire nucleotide sequence of the K2 DNA has been determined. This sequence contains three copies of inverted repeats which are potentially capable of forming hairpin structures. The homology between K2 and K1 DNA as well as with Vicia faba mitochondrial plasmids concerns the area of sequence which may create a hairpin structure. The hybridization experiments revealed homology of plasmid K2 to nuclear DNA of Lupinus angustifolius, but no homology to the main mitochondrial DNA and chloroplast DNA was found. The Northern blot analysis revealed that K1 and probably K2 DNAs are transcribed.

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