Abstract
1.1. Kinetic constants of Discoglossus pictus leucyl-tRNA synthetase were determined. The Km was 4·1 × 10−6 M for leucine, 2·7 × 10−3 M for Mg2+ and 8 × 10−4 M for ATP.2.2. The requirement of Mg2+ ions for each of the two steps of the reaction was investigated. The enzyme adenylate complex was synthesized at a low Mg2+ concentration (⪕5 × 10−7M).3.3. The transfer of leucine on tRNA was activated by Mg2+ up to 10−2 M concentration.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Comparative Biochemistry and Physiology -- Part B: Biochemistry and Molecular Biology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
