Abstract
SUMMARY Hydrangea ringspot virus has been purified to near homogeneity. Virus nucleic acid was identified as single-stranded RNA with an S value of 33.6 before and 21.1 after formaldehyde denaturation. Nucleic acid mol. wt. determinations made by linear log sucrose density gradient centrifugation gave values of 2.25 × 106 and 2.14 × 106, respectively, for native and formaldehyde-treated RNA. Electrophoresis on polyacrylamide gels before and after formaldehyde denaturation gave mol. wt. values of 2.64 × 106 and 2.03 × 106. The values from formaldehyde denatured RNAs are considered the most reliable. Thermal denaturation profiles showed a Tm of 55.2 °C in 0.1 m-sodium phosphate, pH 7.0, and a hyperchromicity of 22.4%.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
