Properties of cytochrome c and methanol dehydrogenase produced by wild type and mutant strains of Methylomonas sp. in continuous culture

Abstract

The cytochrome c production of the wild type strain and a mutant strain, YK 56, of Methylomonas sp. grown with excess methanol was higher than wild with limited methanol. The wild type strain grown under both conditions contained two soluble cytochromes c ( c-I and c-II), though the mutant strain contained three ( c-I, c-II, and c-III). The proportions of cytochromes c-II and c-III of the mutant strain damage changed according to the culture conditions. The methanol dehydrogenase of the wild type and mutant strains was purified and characterized. The enzymes were similar; they consisted of two subunits and their molecular weight was 120,000. The reactivity of cytochromes c with methanol dehydrogenase was investigated.