Abstract

The plasmid pDG101 is indigenous to Corynebacterium flaccumfaciens ssp. oortii and mediates resistance to arsenite, arsenate, and antimony (III). When transferred to Clavibacter (= Corynebacterium) m. ssp. tessellarius, C. m. ssp. nebraskense and C. m. ssp. michiganense, it confers rough colony morphology upon transconjugants. C. m. ssp. transconjugants were less virulent than the smooth recipient strain when inoculated into wheat by partial vacuum infiltration. However, pDG101 did not affect the growth rate of C. m. ssp. tessellarius in the inoculated wheat, nor did it have an effect on in vitro growth rates of either C. m. spp. tessellarius or C. m. ssp. nebraskense. Spontaneous revertants to the smooth colony morphology were obtained from transconjugants both in vitro and in planta. These revertants were sensitive to arsenic and devoid of pDG101. Five d after inoculation of wheat plants with a rough transconjugant strain of C. m. ssp. tessellarius, 0.01% of the reisolated CFU were smooth. Reisolated cells with the rough colony morphology retained resistance to arsenic and harbored pDG101.

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