Abstract
Retention on non-polar chemically-bonded stationary phases depends on the silica carrier, the kind of bonded silane, the surface concentration of bonded alkyl groups and the number of surface silanol groups before and after chemical modification. The influence of carbon content of bonded phases, column temperature and how the eluent mixture has been prepared on solute retention is demonstrated. For comparison of different bonded phases standardization for the amount of bonded carbon per unit column volume is recommended. The problems in attributing specific selectivities to different functional groups in chemically bonded phases are discussed. The advantages and specific selectivities of some phases with functional groups are demonstrated.
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