Abstract
The properties of extracellular cellulase obtained from Bacillus species (FIIRO Strain B223) on supplying cellulose from Brewers Spent Grain (BSG) were studied. Generally, a crude cellulase activity between 11,757 Units to 13,930 Units was observed for both sources of carbon (BSG and crystalline cellulose). The optimum pH and temperature of 5.0, and 30°C - 40°C for both sources of carbon was observed. Heavy metals such as copper, zinc, and iron inhibited the activities of B223 cellulase while the cellulase activities on alkaline earth metal (manganese) were moderate. The co-factor behaviour of manganese ion was also established. Local production of cellulase in Nigeria using local raw materials such as Brewers Spent Grain reduces enzyme cost; cost of finished products and increases gross domestic products.
Highlights
IntroductionThere is an increasing interest in utilising cellulose wastes as feedstock for fermentation processes, thereby
There is an increasing interest in utilising cellulose wastes as feedstock for fermentation processes, therebyHow to cite this paper: Orji, F.A., et al (2016) Properties of Bacillus species Cellulase Produced Using Cellulose from Brewers Spent Grain (BSG) as Substrate
This study reports the use of cellulose from Brewers Spent Grain (BSG) waste for submerged production of cellulase, and the temperature, pH, and metallic ion tolerance properties of the Bacillus cellulase
Summary
There is an increasing interest in utilising cellulose wastes as feedstock for fermentation processes, thereby. How to cite this paper: Orji, F.A., et al (2016) Properties of Bacillus species Cellulase Produced Using Cellulose from Brewers Spent Grain (BSG) as Substrate. Cellulose is mainly degraded by an enzyme known as cellulase. This is mainly produced by bacteria, fungi and protozoan that catalyse cellulolysis (i.e. hydrolysis) of cellulose. There is report of effects of pH, temperature, and metallic ions on cellulase produced from Streptomyces sp., using fruit waste as substrate. This study reports the use of cellulose from Brewers Spent Grain (BSG) waste for submerged production of cellulase, and the temperature, pH, and metallic ion tolerance properties of the Bacillus cellulase
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