Abstract
Rapidly labeled unencapsulated slow-sedimenting RNA (SL-RNA) was synthesized in the presence of actinomycin D by leaf tissue of broadbean infected with broadbean mottle virus (BBMV), but not by healthy leaf tissue. SL-RNA migrated as a definite peak of single-stranded RNA upon electrophoresis in Polyacrylamide gel; its molecular weight was close to, but possibly different from that of the small genomic RNA component which can be extracted from aged BBMV-particles. SL-RNA was not specifically associated with ribosomes; it did not anneal with genomic BBMV-RNA. When total RNA from 4-day BBMV-infected broadbean leaf tissue (devoid of aged BBMV-particles) was centrifuged in sucrose gradient, fractions of the gradient corresponding to the labeled SL-RNA contained nucleotide sequences homologous to genomic BBMV-RNA.
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