Abstract
Rapidly labeled unencapsulated slow-sedimenting RNA (SL-RNA) was synthesized in the presence of actinomycin D by leaf tissue of broadbean infected with broadbean mottle virus (BBMV), but not by healthy leaf tissue. SL-RNA migrated as a definite peak of single-stranded RNA upon electrophoresis in Polyacrylamide gel; its molecular weight was close to, but possibly different from that of the small genomic RNA component which can be extracted from aged BBMV-particles. SL-RNA was not specifically associated with ribosomes; it did not anneal with genomic BBMV-RNA. When total RNA from 4-day BBMV-infected broadbean leaf tissue (devoid of aged BBMV-particles) was centrifuged in sucrose gradient, fractions of the gradient corresponding to the labeled SL-RNA contained nucleotide sequences homologous to genomic BBMV-RNA.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.