Abstract
1. 1. The properties of a soluble ribonuclease from Aedes aegypti larvae have been compared with ribonuclease activity in adult female tissue. 2. 2. In larval extracts ribonuclease activity was maximal at 40–45°C whereas activity in tissue from adult females was highest at 50°C. 3. 3. Ribonuclease activity that was recovered in a 20–60% ammonium sulfate precipitate was further purified by batch elution from DEAE-Sephacel and from carboxymethylcellulose. 4. 4. Ribonuclease activity in the partially purified fraction was sensitive to EDTA, stimulated by magnesium, had a pH optimum at 9.0 and a M r of 45,000. 5. 5. Agarose gels containing yeast RNA substrate were used to monitor partial purification of the larval ribonuclease.
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