Abstract
1. 1. The discovery of a new fast minor Hb component (called “Augusta I”) in cord blood samples of Negro babies, present next to Hb-F, Hb-A and Hb-S, by starch-gel electrophoresis is reported. 2. 2. The amount of the abnormal fraction was found to be about 3% of the total hemoglobin (CMC-chromatography). 3. 3. The component was isolated by CMC-chromatography and studied using different techniques. It showed a slightly increased resistance to denaturation by alkali and an ultraviolet spectrum typical for normal Hb-A. Using starch-gel electrophoresis, the isolated Hb preparation was found to consist of over 90% of the new component next to small amounts of Hb-A and a still faster moving Hb-fraction. 4. 4. It was demonstrated both with Amberlite IRC-50 chromatography and with starch-gel electrophoresis at pH 1.9 that the globin, prepared from the isolated Hbfraction, was composed almost entirely of β polypeptide chains. The formation of Hb-S from a mixture of the new Hb component and Hb-A in hybridization experiments offered strong evidence that this β chain was identical with the abnormal β chain of Hb-S. 5. 5. In the blood of some family members two inherited Hb-abnormalities were demonstrable : a heterozygosity for sickle-cell Hb and the presence of small amounts of a fast moving Hb-fraction, probably identical with Hb-H. These abnormalities were found to be inherited independently.
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