Abstract
Abstract The circularization of a 48 mer oligonucleotide was investigated by using enzymatic ligation with T4 DNA ligase. This method yielded 30% circular molecules. The circular oligonucleotides are molecules with no free ends and are therefore more resistant to exonuclease attack. The circular oligonucleotide has remarkably increased Tm values as compared to the nicked and double stranded DNAs. However, the RNase H activity is lower than that with a single stranded DNA. We also describe the anti-HIV activity of a circular oligonucleotide.
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