Propane sultone mutagenesis in relation to treatment conditions.

Abstract

Effects of concentration, treatment temperature and hydrogen ion concentration on biological activity of 1, 3-Propane sultone (PS) were studied in barley (Hordeum vulgare). Physiological as well as genetic effects of mutagen showed a linear increase with increase in the concentration of mutagen and its treatment temperature. Higher mutagenic effectiveness and efficiency of PS was recorded following treatment with 6 mM and at 25°C. The effect of pH was apparent on root growth injury, as compared to seedling growth damage. A higher mutagenic efficiency was however, recorded at pH=4.6.