Abstract
A protocol for micropropagation of Tribulus terrestris, an important medicinal herb was established using juvenile explants viz., leaf, node and internode. All the explants were tested for callus induction on Murashige and Skoog’s (MS) medium, supplemented with BAP, NAA and 2,4-D. Among the three explants leaf explant responded well (98%) for the callus induction in the MS medium composted with BAP and NAA (4.0 and 0.5 mg/L) followed by the nodal segments (58.75%) in the same medium. Maximum number of shoot induction from the callus of leaf derived explants (91.1%) was perceived on MS medium fortified with BAP 4.0 mg/L and NAA (0.5 mg/L). Moreover, root elongation and profuse rooting percentage (77.19%) were achieved when the well-grown shoots were cultured on MS media supplemented with IAA (2.0 mg/L) for leaf callus derived shoots. The regenerated plantlets were hardened and established at 80% survival rate in hardening media encompassed with red soil, sand and vermicompost in the ratio of 1:1:1 by volume.
Highlights
The genus, Tribulus comprises about 20 species of creeping shrubs or herbs, of which Tribulus terrestris L. (Zygophyllaceae) is the most common
Maximum number of shoot induction from the callus of leaf derived explants (91.1%) was perceived on Murashige and Skoog (MS) medium fortified with BAP 4.0 mg/L and NAA (0.5 mg/L)
The leaf parts of T. terrestris are used to cure renal problems by the tribals of Al-Rass province, Saudi Arabia (Gamal et al, 2010).Juice of fresh leaves is given to animals in case of colic and chronic cough by the rural farmers and traditional herbal healers from Tikamgarh District of Bundelkhand, Central India (Verma, 2014)
Summary
The genus, Tribulus comprises about 20 species of creeping shrubs or herbs, of which Tribulus terrestris L. (Zygophyllaceae) is the most common. It is known as ‘puncture vine’ native of Mediterranean region and that found in warm regions of Europe, Asia, America, Africa and Australia (Frohne, 1999). It is a tap rooted, prostrate, procumbent flowering plant, the stem grows up to 2cm long, leaves are opposite, 4-8 pairs, and spear shaped leaflets, presence of hairs on margin of the leaf. The leaf parts of T. terrestris are used to cure renal problems by the tribals of Al-Rass province, Saudi Arabia (Gamal et al, 2010).Juice of fresh leaves is given to animals in case of colic and chronic cough by the rural farmers and traditional herbal healers from Tikamgarh District of Bundelkhand, Central India (Verma, 2014). The native communities of Terai forest of Western Nepal were stipulated the entire plant is given orally for the treatment of urinogenital tract infection (Anant Gopal et al, 2012)
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