Abstract
Summary and Conclusions(1) Theiler's GD-VII virus has been propagated for 20 passages (transfers) in cultures of chopped mouse embryo brain suspended in ox serum ultranltrate and Simms' X7 solution. After an initial series of culture passages, the cell-free culture fluid killed mice in dilutions as high as 1:1,000,000 when 0.03 cc was injected intracerebrally. Eventually, the same results were achieved when the ox serum ultranltrate was replaced by heated rabbit serum. (2) The virus multiplied more abundantly at 35°C than at 37.5°C; and, the yield was higher in 125 cc Erlenmeyer flasks containing 3 cc of medium than in 50 cc flasks containing 4 cc of medium. (3) No increase in virus output was ever obtained by inoculating subcultures with ground material; nor did grinding the culture tissues improve the mouse titrations. Also, no real advantage was gained by subjecting the cultures to continuous gassing with mixtures of O2 CO2 and N2. (4) The concentration of virus in the culture medium was higher after 2...
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