Abstract
A New Guinea Orchid, Dendrobium antennatum Lindl urgently needs to conserve. The availability of in vitro germination technique for this orchids is significantly important to present the useful tool for conservation effort. This study aimed to find a simple media that consists of Growmore 10-55-10, a foliar fertilizer and complex organic i.e. coconut water, banana pulp, onion spring extract, and carrot juice. Five in vitro medium for seed germination and four in vitro sub-culture medium for planlet growth were formulated and used in this study. Our findings revealed that seed germination in vitro of Dendrobium antennatum well occured on the medium of Growmore 10-55-10 supplemented with 10% coconut water (GCw) and of Growmore 10-55-10 supplemented with 50 g/l extract spring onion. The seed germination process from sowing the seed to plantlet production ready to transfer to sub-culture medium for further growth took time 140 days. The embryo became pale green at the day 11 on the germination media. It could be used as a tool for detection of viability of the seeds. Both seed germination medium could be used also as sub-culture medium for enhancing the growth of the plantlets but not for multiplication of shoots.
Highlights
Dendrobium antennatum Lindl, locally knows as an antelope orchid occurs widely in New Guinea region
Our findings revealed that seed germination in vitro of Dendrobium antennatum well occured on the medium of Growmore 10-55-10 supplemented with 10% coconut water (GCw) and of Growmore 10-55-10 supplemented with 50 g/l extract spring onion
Seed germination is defined as the emergence of radicle through seed coat involving some steps, begins with water imbibition and enzime syntensis followed by embryo growth which develop to protocorm, emergence of the radical, and the production of plantlets (Figure 1)
Summary
Dendrobium antennatum Lindl, locally knows as an antelope orchid (anggrek kelinci) occurs widely in New Guinea region. The species has an economic value aspecially as a parent material in breeding programme to produce new variant of orchids. In its habitat, this orchid species is treatened by the conversion of the forest through the development of the regions. Without any effort this species can be distict, the conservation effort is a must, for example through ex situ conservation. Antennatum easialy to produce fruit with abundance of seeds in nature. It is only a small portion of viable seeds be able to germinate because of its tiny seed. The seed likes these, contains insufficient reserve of nutrients for germination (Teixeira da Silva et al 2015b)
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